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nebnext fast dna  (New England Biolabs)


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    Structured Review

    New England Biolabs nebnext fast dna
    Nebnext Fast Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nebnext+fast+dna+fragmentation+library+prep+set/NEBNext+Fast+DNA+Fragmentation+and+Library+Prep+Set+for+Ion+Torrent/us12410469-212-18-18
    Average 95 stars, based on 90 article reviews
    nebnext fast dna - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Severe COVID-19 induces prolonged elevation of the acute-phase protein pentraxin 3
    Article Snippet: .. Amplicons were enzymatically fragmented (NEBNext Fast DNA Fragmentation & Library Prep Set, New England Biolabs GmbH, Ipswich, MA, USA) and size-selected on an agarose gel (E-Gel EX Agarose Gels, Invitrogen, Thermo Fisher Scientific, Waltham, MA). .. After ligation of sequencing adaptors, purification and selection of sequenceable products, the prepared library was bound to microspheres, and emulsion PCR (ePCR) amplification was carried out using the Ion OneTouch 2 System (Ion Chef System Thermo Fisher Scientific, Waltham, MA).

    Sequencing:

    Article Title: Climate change, circular economy and foodborne pathogens: assessment of pathogenic E. coli and Salmonella spp. in irrigation water in Italy
    Article Snippet: The genomic sequences were produced with Ion Torrent sequencing 340 equipment (Thermo Fisher Scientific, MA, USA). .. Sequencing libraries of about 400 bp were prepared 341 from 100 ng of total DNA using the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion 342 Torrent (New England BioLabs, MA, USA) according to the manufacturer’s instructions. .. 343 The libraries were quantized with the Tape Station System 4200 and the High Sensitivity D1000 344 Reagents kit (Agilent Technologies, CA, USA) and were diluted reaching the concentration of 100 pM 345 in DNAase/RNAase free water for the preparation of a sequencing pool of three samples.

    Article Title: Pathogenic <i>Escherichia coli</i> Virulome in Top Soil Improvers and Irrigation Waters Devoted to Leafy Vegetable Production: Hazard Assessment From a Case Study in Italy
    Article Snippet: The genomic sequences were produced with an Ion Torrent S5 sequencing equipment (Thermo Fisher Scientific, MA, USA). .. Sequencing libraries of about 400 bp were prepared from 100 ng of total DNA using the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent (New England BioLabs, MA, USA) according to the manufacturer's instructions. .. The libraries' concentration was estimated with the TapeStation System 4200 and the High Sensitivity D1000 Reagents kit (Agilent Technologies, CA, USA) and diluted to reach a concentration of 100 p.m. in DNAase/RNAase‐free water for the preparation of a sequencing pool of three samples.

    Article Title: Aeromonas spp. as possible bacterial indicator for monitoring antibiotic resistance in seafood
    Article Snippet: DNA was quantified with the Qubit fluorometer (QubitTM DNA HS Assay, Thermo Fisher Scientific Inc.), the libraries were prepared from 100 ng of DNA using the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent kit (New England BioLabs, MA, USA) and then sequenced using the Ion Chef and Ion Torrent S5 instruments (Thermo Fisher Scientific, MA, USA) using a 400 bp sequencing protocol and the Ion 510 & Ion 520 & Ion 530 kit (Thermo Fisher Scientific, MA, USA). .. DNA was quantified with the Qubit fluorometer (QubitTM DNA HS Assay, Thermo Fisher Scientific Inc.), the libraries were prepared from 100 ng of DNA using the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent kit (New England BioLabs, MA, USA) and then sequenced using the Ion Chef and Ion Torrent S5 instruments (Thermo Fisher Scientific, MA, USA) using a 400 bp sequencing protocol and the Ion 510TM & Ion 520TM & Ion 530 kit (Thermo Fisher Scientific, MA, USA). ..

    Article Title: Characterization of the resistome and antibiotic-resistant bacteria in top soil improvers and irrigation waters devoted to food production: a case study from Italy.
    Article Snippet: DNA was extracted from the filters using the DNeasy PowerWater Kit (QIAGEN, CA, USA) following the manufacturer's instructions (https:// www. qiagen. com/ us/ resou rces/ resou rcede tail? id= 75765 ef9- 2a6f- 4f5d- a36b- dbd9b eb430 79& lang= en). .. Sequencing libraries of 400 bp were prepared from 100 ng of extracted DNA through enzymatic fragmentation by using the NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion TorrentTM Kit (New England BioLabs, MA, Sample code Type Origin ISS2 Manure and animal meal Animal ISS3 Bovine manure Animal USA) according to the manufacturer’s instructions. .. Libraries were quantitated with the Tape Station System 4200 and the High Sensitivity D1000 Reagents kit (Agilent Technologies, CA, USA), diluted to a concentration of 100 pM in DNAase/ RNAase-free water, and pooled in groups of three samples.

    DNA HS Assay:

    Article Title: Aeromonas spp. as possible bacterial indicator for monitoring antibiotic resistance in seafood
    Article Snippet: DNA was quantified with the Qubit fluorometer (QubitTM DNA HS Assay, Thermo Fisher Scientific Inc.), the libraries were prepared from 100 ng of DNA using the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent kit (New England BioLabs, MA, USA) and then sequenced using the Ion Chef and Ion Torrent S5 instruments (Thermo Fisher Scientific, MA, USA) using a 400 bp sequencing protocol and the Ion 510 & Ion 520 & Ion 530 kit (Thermo Fisher Scientific, MA, USA). .. DNA was quantified with the Qubit fluorometer (QubitTM DNA HS Assay, Thermo Fisher Scientific Inc.), the libraries were prepared from 100 ng of DNA using the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent kit (New England BioLabs, MA, USA) and then sequenced using the Ion Chef and Ion Torrent S5 instruments (Thermo Fisher Scientific, MA, USA) using a 400 bp sequencing protocol and the Ion 510TM & Ion 520TM & Ion 530 kit (Thermo Fisher Scientific, MA, USA). ..

    other:

    Article Title: Multiplexed method for the identification and quantitation of minor alleles and polymorphisms
    Article Snippet: A set of experiments was carried using Ion Torrent PGM (Life Technologies) to validate the target regions and to verify the extent of dilutions.

    Article Title: Assessment of Escherichia coli as an indicator of antimicrobial resistance and monitoring of ESBL/AmpC-Producing Enterobacteriaceae in retail seafood commonly consumed in Italy.
    Article Snippet: This study assessed Escherichia coli as an indicator of antimicrobial resistance (AMR) in retail seafood, integrating microbiological and genomic analyses within a One Health perspective.. Overall, E. coli was cultured from 55 (13%) of 422 raw samples from seven seafood categories commonly consumed in Italy.. E. coli was detected across multiple seafood types, but particularly in bivalves (23%) and crustaceans (20%).

    Enzymatic Fragmentation:

    Article Title: Characterization of the resistome and antibiotic-resistant bacteria in top soil improvers and irrigation waters devoted to food production: a case study from Italy.
    Article Snippet: DNA was extracted from the filters using the DNeasy PowerWater Kit (QIAGEN, CA, USA) following the manufacturer's instructions (https:// www. qiagen. com/ us/ resou rces/ resou rcede tail? id= 75765 ef9- 2a6f- 4f5d- a36b- dbd9b eb430 79& lang= en). .. Sequencing libraries of 400 bp were prepared from 100 ng of extracted DNA through enzymatic fragmentation by using the NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion TorrentTM Kit (New England BioLabs, MA, Sample code Type Origin ISS2 Manure and animal meal Animal ISS3 Bovine manure Animal USA) according to the manufacturer’s instructions. .. Libraries were quantitated with the Tape Station System 4200 and the High Sensitivity D1000 Reagents kit (Agilent Technologies, CA, USA), diluted to a concentration of 100 pM in DNAase/ RNAase-free water, and pooled in groups of three samples.



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